Affinity Chromatography of RNA-Dependent DNA Polymerase from RNA Tumor Viruses on a Solid Phase Immunoadsorbent

Affinity chromatography of RNA-dependent DNA polymerase from RNA tumor viruses on a solid phase immunoadsorbent.

A solid phase immunoadsorbent specific for RNA-dependent DNA polymerase from murine and feline RNA tumor viruses has been prepared. The enzymes from murine and feline virus but not from avian virus bind to columns of this material. Bound enzymes can be eluted in active form. This method has permitted selective purification of viral enzyme from crude extracts of virus-transformed cells, since th...

An oligonucleotide affinity column for RNA-dependent DNA polymerase from RNA tumor viruses.

Columns of (dT)(12-18)-cellulose provide a one-step enrichment procedure for RNA-dependent DNA polymerase. The enzyme of the virus from RD-114 cells, as well as that from Rauscher murine leukemia virus, have been purified in this way. The preference of viral as compared to cellular DNA polymerases for (dT)(12-18) as a primer is reflected in the fact that the DNA polymerases of uninfected cells ...

RNA-dependent DNA polymerase in virions of RNA tumour viruses.

Enhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase

Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...

Antibody to the RNA-dependent DNA polymerase of mammalian C-type RNA tumor viruses.

Sera from rats bearing transplantable tumors induced by murine C-type tumor viruses contain an inhibitor of the activity of the viral RNA-dependent DNA polymerase. The inhibitor is shown to be an immunoglobulin (IgG) directed against the enzyme. Antiserum made in rabbits against partially purified murine leukemia virus polymerase also inhibits the polymerases of other mammalian C-type RNA-conta...